Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer
Artículo de revista
2019-09
John Wiley and Sons, Inc
Journal of Cellular Physiology
235
4
3320
3328
0021-9541
1097-4652
Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes
approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced
stages resulting in a high mortality rate. The objective of the study was optimizing the
isolation of cells obtained from the solid tumor and ascitic fluid of patients
with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and
cultivated with different methodologies. As a result, cell growth was obtained and
epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM,
N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor
was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in
terms of the expression of mesenchymal and epithelial type markers in the two types
of isolated cells. Additionally, CD133 and CD44
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