Envíos recientes

D. Facultad de Medicina

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Generalidades de paciente adulto con adenopatías

Walter Gabriel Chaves Santiago | 2021

Al enfrentarse a un paciente adulto que consulta por adenopatías, hay varios aspectos importantes en la anamnesis y en el examen físico a tener en cuenta como las características de adenopatías, si hay o no síntomas que acompañen a la aparición de las adenopatías y los antecedentes del paciente. Si se realiza una adecuada interpretación de lo obtenido en el interrogatorio y en el examen físico, se podrá establecer una impresión diagnóstica y a vez esto permitirá solicitar los paraclínicos pertinentes en cada caso para poder definir el diagnóstico.

BAX326 (RIXUBIS): a novel recombinant factor IX for the control and prevention of bleeding episodes in adults and children with hemophilia B

Solano Trujillo, María Helena | 2014

Abstract: Hemophilia B management has improved considerably since the introduction of high-purity plasma-derived factor IX (pdFIX) products in the early 1990s. Recombinant FIX (rFIX) was introduced more recently and has potential safety advantages over the older bloodbased products. Until recently, only one such product, nonacog alfa (BeneFIX®, Pfizer, Inc.), has been available. However, a new rFIX product, BAX326 (RIXUBIS, Baxter Healthcare Corp.), has now been approved by the US Food and Drug Administration. BAX326 undergoes rigorous virus elimination and purification steps during manufacture, and has low activated FIX activity, which confers low thrombogenic potential in humans. Preclinical studies showed promising pharmacokinetic and safety profiles, and these early findings have since been expanded in a series of prospective, multicenter, clinical studies. Foremost among these is a pivotal phase I/ III study of BAX326 and its use in routine prophylaxis or on-demand treatment in patients aged 12–65 years with severe (FIX level <1%) or moderately severe (FIX level ⩽2%) hemophilia B. This study confirmed the pharmacokinetic equivalence of BAX326 and nonacog alfa, and showed a significant reduction in annualized bleeding rate with BAX326 prophylaxis compared with on-demand treatment (79% versus historic controls; p<0.001). The hemostatic efficacy of BAX326 was rated as ‘excellent’ or ‘good’ in 96% of bleeds. BAX326 was also associated with statistically significant and clinically meaningful improvements in physical health-related quality of life. Results are similarly encouraging in a pediatric study in children aged up to 12 years and in a study in hemophilia B patients undergoing surgery. A further study showed safe transition, with no inhibitor formation in any patient, from treatment with a pdFIX product to BAX326. Overall, the safety profile of BAX326 in clinical trials has been strong, with no inhibitor or specific antibody formation, thrombosis, or treatment-related serious adverse events or anaphylaxis.

Lemierre’s Syndrome Caused by Streptococcus anginosus Presenting as Postseptal Cellulitis in a Pediatric Patient

Camacho Cruz, Jhon | 2019

Lemierre’s syndrome is an infrequent disease characterized by septic thrombosis of the internal jugular vein followed by pulmonary embolism generally occurring after upper respiratory and gastrointestinal tract infections. We present the case of a 15-year-old female patient with postseptal cellulitis and cervical abscess who developed pulmonary embolism and pleural effusion secondary to internal jugular vein thrombosis. Cultures were positive for Streptococcus anginosus, antibiotic treatment was established with satisfactory clinical outcome. High clinical suspicion is required for a diagnosis. The mainstay of treatment is a multidisciplinary approach based on two essential pillars: antibiotic therapy and surgical drainage. This is an important case because of the unusual presentation, the isolation of an infrequent pathogen, and the primary infection site (postseptal cellulitis), which are rare characteristics of this condition in the pediatric population.

Colonic adenosquamous carcinoma and mucinous adenocarcinoma with microsatellite instability

Parra Medina, Rafael | 2018

A 43-year-old man presented with two-month history of fatigue, weakness, paleness, rectal bleeding, sweating, and weight loss of 10 kg in the past one month. A complete blood count revealed anaemia. The patient underwent a right hemicolectomy. The microscopic examination revealed an adenosquamous carcinoma associated with a mucinous adenocarcinoma in a patient with microsatellite instability due to loss of MLH1 and PMS2 expression and retention of MSH2 and MSH6 expression in both the squamous and glandular components. We also observed an atypical immunohistochemical phenotype in the adenocarcinoma component showing CK7 expression and reduced CK20 and CDX2 expression

Multiplatform plasma metabolic and lipid fingerprinting ofbreast cancer: A pilot control-case study in ColombianHispanic women

Cala, Mónica P. | 2018

Breast cancer (BC) is a highly heterogeneous disease associated with metabolic reprogramming. The shifts in the metabolomecaused by BC still lack data from Latin populations of Hispanic origin. In this pilot study, metabolomic and lipidomic approacheswere performed to establish a plasma metabolic fingerprint of Colombian Hispanic women with BC. Data from H-NMR, GC-MSand LC-MS were combined and compared. Statistics showed discrimination between breast cancer and healthy subjects on allanalytical platforms. The differentiating metabolites were involved in glycerolipid, glycerophospholipid, amino acid and fatty acidmetabolism. This study demonstrates the usefulness of multiplatform approaches in metabolic/lipid fingerprinting studies to broadenthe outlook of possible shifts in metabolism. Our findings propose relevant plasma metabolites that could contribute to a betterunderstanding of underlying metabolic shifts driven by BC in women of Colombian Hispanic origin. Particularly, the understanding ofthe up-regulation of long chain fatty acyl carnitines and the down-regulation of cyclic phosphatidic acid (cPA). In addition, themapped metabolic signatures in breast cancer were similar but not identical to those reported for non-Hispanic women, despiteracial differences.

Identifcation of candidate miRNAs in early‑onset and late‑onset prostate cancer by network analysis

| 2020

The incidence of patients under 55 years old diagnosed with Prostate Cancer (EO-PCa) has increased during recent years. The molecular biology of PCa cancer in this group of patients remains unclear. Here, we applied weighted gene coexpression network analysis of the expression of miRNAs from 24 EO-PCa patients (38–45 years) and 25 late-onset PCa patients (LO-PCa, 71–74 years) to identify key miRNAs in EO-PCa patients. In total, 69 diferentially expressed miRNAs were identifed. Specifcally, 26 and 14 miRNAs were exclusively deregulated in young and elderly patients, respectively, and 29 miRNAs were shared. We identifed 20 hub miRNAs for the network built for EO-PCa. Six of these hub miRNAs exhibited prognostic signifcance in relapse‐free or overall survival. Additionally, two of the hub miRNAs were coexpressed with mRNAs of genes previously identifed as deregulated in EO-PCa and in the most aggressive forms of PCa in African-American patients compared with Caucasian patients. These genes are involved in activation of immune response pathways, increased rates of metastasis and poor prognosis in PCa patients. In conclusion, our analysis identifed miRNAs that are potentially important in the molecular pathology of EO-PCa. These genes may serve as biomarkers in EO-PCa and as possible therapeutic targets.

Cost of Patients With Hemophilia A and High-Titer Inhibitors in Colombia

Sánchez Vanegas, Guillermo | 2019

Background: In Colombia, hemophilia is considered a high-cost disease, and hemophilia A with high-titer inhibitors may be responsible for a significant economic pressure on the Colombian health system. Objectives: To estimate the direct cost of care for patients with hemophilia A with high-titer inhibitors in Colombia, from the perspective of the health system. Methods: A cost-of-illness study was carried out using standard case methodology, which was designed based on literature review and validation by expert consensus. Scenarios were established for adults and children, including cases of prophylaxis, immune tolerance induction, bleeding, and surgery. The frequencies were taken from the official report for Colombia, issued by the HighCost Account 2017 (reported 2018). The prices were obtained from the list of regulated medicines in the country. The cost estimate is presented with a range of values by weight (between 10 kg and 90 kg).Results: The total estimated cost per year for Colombia was US $44 905 252 (between US $32 260 497 and US $58 202 393). The average cost per year calculated for a patient was US $498 947 (between US $358 450 and US $646 693). A total of 99.8% of the estimated cost was directly related to the cost of the coagulation factors and bypassing agents. Conclusions: Hemophilia A with high-titer inhibitors is a disease that generates significant pressure on the Colombian health system, mainly linked to the cost of factors and bypassing agents.

Spanish Translation, Cultural Adaptation, and Validation of the Standardized Cosmesis and Health Nasal Outcomes Survey Questionnaire

Pérez García, Irene C. | 2019

Background: The Standardized Cosmesis and Health Nasal Outcomes Survey (SCHNOS) questionnaire is a new instrument that was developed to evaluate both functional and cosmetic components of rhinoplasty. It is a reliable, consistent, and validated patient-reported outcome measure that is not available in Spanish. Methods: The SCHNOS questionnaire was forward translated, back translated, and culturally adapted following international guidelines. Its psychometric validity was tested with native Spanish speakers in 2 centers in Colombia. The authors measured internal consistency, correlation, and reproducibility to determine validity of the instrument. Results: The final Spanish version of the SCHNOS was administered to 76 native Spanish speakers. Both the SCHNOS-O (obstructive domain) and SCHNOS-C (cosmetic domain) showed a high internal consistency with Cronbach’s alpha of 0.84 and 0.94, respectively. The Spearman correlations between the items of SCHNOS-O (0.38–0.82) and SCHNOS-C (0.49–0.88) were positive and significant. Spearman’s rank correlation in the test–retest analysis for SCHNOS-O (r = 0.87) and SCHNOS-C (r = 90) was positive and statistically significant. There was statistical significance in responses obtained for SCHNOS-O (P < 0.001) but not for SCHNOS-C (P = 0.222). Conclusions: In this study, the SCHNOS was successfully translated and culturally adapted into Spanish. The Spanish version of the SCHNOS was shown to be a reliable and valid instrument that we recommend it should be used in Spanishspeaking patients who are having functional or cosmetic rhinoplasty.

Calidad de la comunicación con el cuidador del paciente pediátrico

Tolosa, D. | 2018

Introducción: Las deficiencias en la comunicación médico-paciente repercuten en el proceso de atención y en el desenlace terapéutico. El objetivo de este estudio fue determinar la percepción de la información suministrada por los médicos tratantes por parte de los padres o cuidadores principales y los déficits observados en su relación durante la hospitalización de pacientes pediátricos en unidades de alta, media y baja complejidad. Materiales y métodos: Estudio descriptivo realizado desde enero a mayo de 2017 en la Fundación Hospital de la Misericordia de Bogotá (Colombia), a través de una encuesta anónima, autodiligenciada durante la estancia hospitalaria, conformada de una primera parte de preguntas abiertas, y la segunda por una encuesta tipo Likert con variables cualitativas. Las variables cualitativas evaluadas a través de la encuesta se resumieron en frecuencias; además, se realizó la prueba de Kaiser-Meyer-Olkin con análisis de componentes principales bajo una rotación Varimax. Resultados: Se encuestaron 387 cuidadores principales, 68,5% se encontraban en estancia general, 20,4% en cuidados intermedios y 11,1% en cuidados intensivos. El 90% de los encuestados estaban de acuerdo en la forma en que se explicaba la evolución del paciente y expresaron su satisfacción acerca de la información entregada, el lenguaje utilizado y el interés hacia sus inquietudes. Sin embargo, el 41% manifestaron incertidumbre por la evolución médica de su hijo. Conclusiones: Se encontró una percepción adecuada respecto a la información médica suministrada, el lenguaje empleado, el trato hacia el paciente y la ensenanza ˜ al cuidador principal sobre la enfermedad que presentaba el paciente. La incertidumbre con respecto a la evolución de los pacientes es el factor aparente que contribuye en mayor medida a la insatisfacción con la calidad de la información.

Características clínicas de pacientes con mastocitosis cutánea pediátrica en dos hospitales de Bogotá-Colombia

Novoa, Mónica | 2019

Objetivo: Describir las características clínicas de pacientes pediátricos con mastocitosis cutánea atendidos en dos hospitales de Bogotá durante un período de 6 años (2012-2017). Material y métodos: Estudio de tipo serie de casos. Se revisó historias clínicas de los pacientes pediátricos obteniéndose datos clínicos como edad de aparición, sexo, antecedentes personales y familiares, localización de las lesiones, compromiso sistémico, resultados de exámenes de laboratorio, ecografía abdominal y biopsia de piel. Se realizó estadística descriptiva obteniéndose frecuencias, porcentajes, medidas de tenencia central y de dispersión. Resultados: Se incluyó a 24 pacientes; 9 mujeres y 15 hombres. La mediana de edad de aparición fue de 2 meses con un valor máximo de 60 meses. La variante clínica más frecuente fue la mastocitosis maculopapular (54,2%), seguida del mastocitoma solitario (41,7%) y mastocitosis cutánea difusa (4,2%). El signo de Darier fue positivo en la mayoría de los casos (83,0%), se observó una muy baja frecuencia de compromiso sistémico (4,2%). Las lesiones se localizaron predominantemente en tórax y abdomen (54,2%), recibiendo en su mayoría manejo expectante (50%). La biopsia fue realizada en el 54,2% con resultado confirmatorio, se realizó estudios de extensión en el 29,2% y se empleó manejo intrahospitalario en la variante cutánea difusa. Conclusiones: La mastocitosis cutánea en los hospitales estudiados es más frecuente en el sexo masculino con predominio de la forma clínica maculopapular, alta frecuencia de lesiones en tórax y abdomen, signo de Darier positivo y muy baja frecuencia de compromiso sistémico.

Expression of heat shock protein Hsp 27 in ovarian carcinoma

Gutiérrez Castañeda, Luz Dary | 2020-04

Objective: The aim of the present study was to determine the expression of heat shock protein 27 (Hsp 27) in patients with ovarian carcinoma and its relationship with clinical and histopathological characteristics. Materials and Methods: Cross-sectional study in patients diagnosed with ovarian carcinoma. From paraffin blocks with tumor material, archived in the pathology services of the San Jose and Infantil Unversitario de San Jose hospitals since 2013 to 2016 the expression of Hsp 27 was analyzed using a semi-quantitative measurement method. Patients´ clinical records were reviewed to analyze clinical and histopathological characteristics. Results: Immunohistochemistry was performed on 31 patients. Hsp 27 immunostaining was positive in more than 50% of tumor cells in 17 (54.8%) cases. The intensity of Hsp 27 expression was strong in 13 (41.9%) cases. The most frequent location was membranous and cytoplasmatic in 25 (80.6%). The characterization of patients with intensity of 3+ occurred mainly in high-grade serous papillary stage IIIC carcinomas. Conclusion: A stronger Hsp 27 expression occurred mainly in patients with high-grade serous, histology, advanced FIGO stage and in whom optimal or complete cytoreduction could not be performed.

Intraretinal hemorrhages in newborns: Incidence and associated factors

Solano, Adriana | 2016

Purpose: To determine the incidence of intraretinal hemorrhage in newborns at the Hospital de San José, Bogotá, Colombia from September 2009 to September 2010. Design: Cohort study. Methodology: Live newborns were examined at the San José Hospital for a year (September 2009 to 2010), analyzing neonatal and maternal factors associated with the presence of intraretinal hemorrhages. A simple random cluster sampling was used, excluding newborns with a compromised general status, as well as those whose parents or legal guardians did not sign the informed consent form. Patients with the presence of intraretinal hemorrhages were examined every 2 weeks until resolution was observed. Results: A total of 190 newborns were examined, finding an incidence of intraretinal hemorrhages of 22.6% (95% CI 16.6 to 28.6). Most of the mothers were nulliparous (55.8%) and deliveries were eutocic (65.1%). From newborns with hemorrhages, 81.3% were born at term, and hemorrhages in most patients (93.0%) were resolved by the second week of monitoring. Associations between hemorrhages and cesarean section (RR 0.42, CI 0.22 to 0.79) and episiotomy (RR 1.73, CI 1.02 to 2.94) were observed. Conclusions: Newborns who presented intraretinal hemorrhages were children of nulliparous mothers on whom episiotomy was performed, and some with eutocic and instrumenteddeliveries. The first two variables had statistical significance for the development of intraretinal hemorrhages.

Implementación de un protocolo de entrenamiento orientado en pacientes adultos en unidad de cuidados intensivos. Reporte de casos

Enciso, César | 2020-08-14

Introducción: La inmovilización prolongada en la Unidad de Cuidados Intensivos (UCI) es un factor independiente, que se asocia con la aparición de severas alteraciones neuromusculares, agrupadas bajo el concepto de debilidad asociada a UCI (DA-UCI) . Entre ellas: el síndrome de desacondicionamiento físico (SDF), diferentes formas de poli neuropatía y atrofia muscular, son los más frecuentes. La asociación de estas alteraciones con cambios metabólicos, mayor probabilidad de infección y mayor estancia en UCI y estancia hospitalaria, han orientado a la búsqueda de las mejores estrategias de prevención y tratamiento. Se ha demostrado con la evidencia actual, un resultado favorable de la movilización temprana y se presenta una modificación a los protocolos de rehabilitación con base en el concepto de entrenamiento orientado en un grupo de pacientes adultos críticamente enfermos. Objetivo: Describir el resultado, de la implementación de un protocolo de entrenamiento orientado a pacientes adultos en cuidados intensivos.Metodología: Se seleccionaron pacientes adultos con más de 48 horas de ventilación mecánica a quienes a diario, previa evaluación de sus condiciones cardiovasculares y ventilatorias se implementó un protocolo de entrenamiento, dirigido por un médico deportólogo, quien seleccionó la carga según su evaluación antropométrica y fase de rehabilitación previo al inicio de la sesión.Se registró la información relacionada con las variables hemodinámicas y ventilatorias, así como las barreras encontradas para la implementación. La evaluación funcional se presenta con el puntaje de la escala de Barthel, realizada al inicio y al final de la intervención Resultados: Se intervinieron 6 pacientes, con predominio de sexo femenino con un 66,6 %, rango de edad entre 20 y 89 años y una estancia en UCI de 4 a 114 días, cada paciente recibió entre 2 y 16 sesiones con cargas hasta el 40%, con un puntaje en escala de Borg modificado de 4 a 6. Un total de 13 sesiones suspendidas por factores tales como dolor, fatiga física, sedación, trastornos electrolíticos, taquicardia en reposo. Conclusión: Un protocolo de entrenamiento en pacientes críticos no mostró ser un factor desencadenante de alteraciones en las variables cardiovasculares y ventilatorias por lo que se puede considerar una estrategia segura. El dolor, el requerimiento de recursos adicionales, la sedación nocturna, son los factores de mayor frecuencia relacionados con la suspensión del protocolo.

Right hepatectomy after spontaneous hepatic rupture in a patient with preeclampsia: A case report

Millan, Carlos A. | 2017-08

INTRODUCTION: Spontaneous hepatic rupture associated with preecalmpsia or HELLP syndrome is a rare and life threatining event, only 200 cases have been reported in the literature. PRESENTATION OF CASE: We present a case of a 31 year old female with 28 weeks of gestation that presented with acute abdominal pain, elevated blood pressure and altered liver enzymes an abdominal ultrasound that showed a subcapsular hematoma occupying the whole right lobe and free abdominal fluid, she required emergent laparotomy, C-section, hepatic packing, followed by angioembolization and finally right hepatectomy. DISCUSSION AND CONSLUSION: Spontaneous hepatic rupture due to preeclampsia or HELLP syndrome is a medical emergency, it requires a prompt and decisive treatment. Multiple treatment modalities are available, from simple hepatic packing to endovascular embolization, but in extreme situations a formal hepatectomy might be required.

Evaluation of visual function in patients with cataract, amblyopia, optic neuritis, and retinopathies

Espinosa, Natalia | 2020-08

Purpose: The purpose was to describe the changes in the visual function of patients with cataract, optic neuritis, amblyopia, and retinopathies evaluated at the Hospital de San José, Bogotá, Colombia, between August 2015 and May 2019. Materials and Methods: This is a descriptive cross‑sectional study. One hundred and ninety‑six patients with a diagnosis of cataract, retinopathies, optic neuritis, and amblyopia were evaluated; the tests were performed to determine the visual function (visual acuity, stereopsis, contrast sensitivity, and color tests). Qualitative and quantitative characteristics were described. Results: Of the 196 patients, 17.3% had amblyopia of any cause, 16.8% optic neuritis, 40.8% cataract, and 25% retinopathies of multiple etiologies. Amblyopic patients presented less deterioration of the stereopsis(160 arc seconds), whereas patients with retinopathy presented more significant compromise (400 arc seconds). Patients with optic neuritis showed severe deterioration in contrast sensitivity; in the other pathologies, the decline was moderate. Nearly 42.4% of the patients with optic neuritis and 38% of the amblyopic patients did not present color vision disorders, whereas 32.6% and 31.2% of the patients with retinopathy and cataract showed tritanomaly. Conclusion: Visual acuity is not equivalent to visual function. Stereopsis, contrast sensitivity, and color vision are affected by pathologies such as amblyopia, optic neuritis, cataract, and retinopathies. These assumptions of visual function could be considered for studies that take into account all the factors that may compromise the visual performance and, accordingly, define early diagnosis strategies, in addition to therapeutic behaviors such as performing early cataract surgery and visual rehabilitation.

Pediatric ocular myasthenia gravis: Case report and literature review

Jimeno, Verónica | 2019-12

Objective: The objective was to describe a case of ocular myasthenia gravis (MG) in a pediatric patient. Design: This is a descriptive, retrospective study and case report. Methodology: For the purpose of the case report, we searched and analyzed the literature referring to the diagnosis and treatment of ocular MG (OMG) in children. Description: A previously healthy 5‑year‑old girl presented to the emergency room because of sudden‑onset ptosis of the right upper lid without other symptoms. Her examination demonstrated fluctuating ptosis and limitation to downgaze in the right eye. Because of the fluctuation of the ptosis, OMG was suggested, and the patient was tested with ice‑pack test and neostigmine test with positive results. Treatment with pyridostigmine was initiated with a good response. Discussion: OMG has a very low incidence in pediatric patients. It is a disorder characterized by impairing neurotransmission at the neuromuscular junction that generates extraocular muscle weakness. It is important that ophthalmologists have knowledge about this disease because it is a differential diagnosis when there is a patient who has ptosis, strabismus, or limitation of movements. There are no standard diagnostic criteria for this disease; the diagnosis is based on clinical presentation and pharmacologic, serologic, and electrophysiologic tests. The treatment is based on steroids and anticholinesterase drugs. Some patients will require plasmapheresis or immunomodulators.

Is it prudent to consent to or recommend pregnancy in breast cancer survivors?

Monterrosa Blanco, Angélica | 2020-06

Background: Female breast cancer survivors and some health professionals may have doubts about the advisability of pregnancy after breast cancer. Such doubts concern possible recurrences, increased mortality, and adverse effects on the newborn. Purpose: The aim of this report is to present the case of a woman who, after 48 months of breast cancer survival, wished to start a pregnancy. A further aim is to indicate whether it is prudent to consent to or to recommend pregnancy after a breast cancer diagnosis. Case report: This report concerns a patient without satisfied parity, who, at the age of 36 years, presented left breast carcinoma with negative nodes: estrogen receptors in less than 2% of the studied cells and negative progesterone receptors. She received surgical management, chemotherapy and radiotherapy. At 48 months of survival, she wished to start a new pregnancy. On requesting a risk/benefit recommendation from her treating doctors, she did not obtain a unanimous position. By her own decision, she sought and achieved a spontaneous pregnancy, which led to a live birth. Five years later, her child shows normal growth. Conclusion: Evidence suggests increased survival in mothers with a breast cancer history and subsequent pregnancy. Despite being high-risk pregnancies due to the increased possibility of low birth weight, preterm delivery, and small fetus for gestational age, studies suggest that pregnancy may be consented to or recommended in breast cancer survivors.

Septic embolism: the importance of an adequate clinical approach: a case report, Bogota, Colombia

Clavijo Prado, Carlos Andrés | 2016-06

Neurological complications of infectious endocarditis have a high prevalence globally and its clinical presentation remains a clinical challenge. A previously healthy 36-year old male presented at the emergency room with persistent fever and headache. The patient was referred to Neurology section after initial diagnostics and treatment. They detected abnormal skin lesions and cardiac murmur. Based on the presentation and abnormal physical examination findings, their impression was an embolic process with infectious etiology prompting medical treatment. Patient was concomitantly referred to Cardiology section and infective endocarditis was confirmed by echocardiogram. Cranial Magnetic Resonance Imaging (MRI) confirmed an ischemic embolic process. Successful surgical treatment followed. This highlights the importance of good clinical history and physical examination as the cornerstone of diagnosis, even for the clinically challenging cases. Good clinical approach and appropriate use of imaging technology greatly benefited the patient by allowing early diagnosis and treatment hence prevention of further complications.

Xeno-free extraction, culture, and cryopreservation of human adipose-derived mesenchymal stem cells

Escobar, Carlos Hugo | 2016-02

Molecules of animal or bacterial origin, which pose a risk for zoonoses or immune rejection, are com-monly used for extraction, culture, and cryopreservation of mesenchymal stem cells. There is no se-quentialandorderlyprotocolforproducinghumanadipose-derivedstemcells(hASCs)underxeno-freeconditions. Afterstandardizinga humanplateletlysate (hPL) productionprotocol,four humanadiposetissue samples were processed through explants with fetal bovine serum (FBS)-supplemented or hPL-supplemented media for extracting the adipose-derived stem cells. The cells were cultivated in cellculture medium + hPL (5%) or FBS (10%). The cellular replication rate, immunophenotype, and differ-entiation potential were evaluated at fourth passage. Cellular viability was evaluated before and aftercryopreservation of the cells, with an hPL-based solution compared with an FBS-based solution. Theexplants cultured in hPL-supplemented media showed earlier and faster hASC proliferation than didthosesupplementedwithFBS.Likewise,cellsgrowninhPL-supplementedmediashowedagreaterpro-liferation rate, without losing the immunophenotype. Osteogenic differentiation of xeno-free hASCwas higher than the hASC produced in standard conditions. However, adipogenic differentiationwas reduced in xeno-free hASC. Finally, the cells cryopreserved in an hPL-based solution showed ahigher cellular viability thanthecells cryopreserved inanFBS-based.In conclusion, we have developeda complete xeno-free protocol for extracting, culturing, and cryopreserving hASCs that can be safelyimplemented in clinical studies.

Correlación del Queen's College Step Test y Ergoespirometría para estimación del VO2 max

Galvis Rincón, Juan Carlos | 2020-04

El sedentarismo es un factor de riesgo cardiovascular de gran importancia, por lo que conociendo el nivel de consumo de oxígeno máximo (VO2max), se pueden realizar mejoras en los niveles de actividad física y del ejercicio. El objetivo de este estudio es determinar la relación que existe entre la ergoespirometría y Queen’s College Step Test en hombres sanos y físicamente activos de los programas deportivos de una universidad de Bogotá – Colombia, debido a que el Queen’s College Step Test no ha sido validado en población colombiana. Los participantes fueron 52 hombres (Rango edad 17-35 años) a quienes se les realizaron cada una de las pruebas; se analizaron los resultados aplicando el coeficiente de correlación de Pearson, el cual fue baja con un r=0.278; generando una correlación poco significativa y una recomendación desfavorable en esta población.